Essential when working with low-abundance samples.
Rinsing with a buffer like 0.1% trifluoroacetic acid (TFA). Essential when working with low-abundance samples
Using a high-organic solvent to release the purified protein directly onto a MALDI plate or into a fresh tube. Real-World Application: Mass Spectrometry Essential when working with low-abundance samples
Preparing the resin with a wetting solution (e.g., 50:50 acetonitrile in water). Essential when working with low-abundance samples
Allows for desalting and concentrating samples in a single, fast step. Standard Workflow for Sample Prep
Using these tips typically follows a simple four-step process: